P-348 The contribution of circulating tumor cells’ gene expression in treatment response

Colorectal cancer, constitutes one of the most prevalent types cancer. Despite various treatment options, mortality rate is extremely high. Chemotherapy and/or radiotherapy might be beneficial for patients, however “indicators” which could predict response to above protocols, would helpful. Study circulating tumor cells (CTCs), in combination with high-sensitivity assays, contribute more successful protocols. In this study, we performed gene expression analysis CTCs derived from patients colorectal cancer and compared outcome established Blood sample was isolated 54 at different stages disease. detected Fluorescence-Activated Cell Sorting (FACS). RNA Isolated qRT-PCR followed than 90 related genes. The genes included growth factors receptors, self-repair mechanisms, angiogenesis, cell cycle regulation, apoptosis, metastasis, drug metabolism as well specific biomarkers. Gene also non-cancer samples, relative quantification followed. Among were overexpressed, transcription involved signal transduction pathways (FOS, JUN, JAK2), apoptosis (FAS, BAX), (TYMS, PNP, DPYD), regulation (CDKN1B). On contrary, encoding heat shock proteins downregulated (HSP90AA1, HSPA1A, HSPB1). These preliminary data indicate that not all cases exhibit same behavior, it expected. There are overexpressed genes, whose associated chemotherapeutic drugs, like fluorouracil, oxaliplatin, irinotecan, monoclonal antibodies, such nivolumab, cetuximab etc. However, these upregulated cases, since there a percentage approximately 30% profile. almost showed sensitivity hyperthermia. Concluding, has been demonstrated important comprehensive design effective